The recognition that a previously known anti-malarial, antibiotic azithromycin, has an additional novel mode of action, that of inhibiting invasion of red blood cells, should spur on the development of azithromycin derivatives with improved properties.
The reported drop in mortality from malaria in the past decade from over a million to just under 600,000 per year has been attributed to better diagnosis, the use of insecticide-treated bednets, and treatment with artemisinin-based combination therapies. However, concern regarding the sustainability of the latter is based on reports of growing resistance to artemisinin by Plasmodium falciparum (the malaria parasite that is responsible for the majority of deaths from malaria worldwide) that is occurring in the Greater Mekong sub-region. The search for new antimalarial drugs is ongoing.
In the past, most antimalarial drugs have been directed at the parasite as it develops inside red blood cells (RBCs). However, the malaria parasite is probably at its most vulnerable just before it invades a new RBC as it is directly exposed to agents circulating in the blood.
RBC invasion occurs very rapidly after the release of the invasive stages, called merozoites, from previously infected RBCs. Invasion is a multistage process involving initial attachment to an RBC and orientation so that the apical end is touching the cell. A tight junction forms between the merozoite and the RBC and inward movement is driven by an actinomyosin motor. These stages require an array of proteins present on the surface of the merozoite and secreted from internal organelles called rhoptries.
No drug has been developed so far that would inhibit any aspect of this invasion process. However, a report recently published by Danny Wilson and colleagues suggests this is now feasible. The recent development of methods to collect and purify merozoites and to quantify invasion using flow cytometry have enhanced the ability to study the effect of potential antimalarial drugs on the invasion of RBCs in vitro and enabled this group to discover a new mode of action for a previously recognized group of macrolide antibiotics that act on the RBC stage of the parasite.
Two macrolide antibiotics, in particular, azithromycin and clindamycin, have a good safety record for use with children and pregnant women and have an extremely long half-life in vivo. They were already known to inhibit the synthesis of proteins in an organelle of the parasite called the apicoplast and to lead to death in a delayed manner whereby the parasite can complete one cycle of division inside an RBC but dies during the following cycle.
Focusing on azithromycin, the group applied the antibiotic to parasites in culture with RBCs. They monitored its effect when added at different phases of the first and second cycle of invasion and growth, with or without washing away the antibiotic. Both flow cytometry and microscopy were used to observe the results. They also filmed the invasion of new RBCs when the drug was applied just before the release of a new batch of merozoites.
Comparison of the inhibitory effect of the drug dissolved in 2 different vehicles showed that, when DSMO was used as a vehicle, the killing effect was 5-fold greater when the assay examined its effect on invasion than when the previously recognized delayed effect on growth was monitored. This evidence suggested the azithromycin had two different modes of action.
Both flow cytometry and microscopy were used to demonstrate that when merozoites were exposed to azithromycin they were unable to invade new RBCs and no ring stages developed in these cells. Furthermore, by comparing the exposure of merozoites to the antibiotic for 10 mins with exposure of RBCs for one hour, the group demonstrated that it was the interaction of a merozoite with an RBC that was inhibited, rather than an event that occurred immediately after they invaded the cells and then encountered the drug.
Thus, in addition to its effect on the apicoplast, azithromycin also stopped merozoites from invading new RBCs, thereby preventing new cycles of parasite multiplication.
Live cell imaging showed that azithromycin treatment affected the early stage of merozoite invasion when contact is first made with the RBC, preventing the formation of a tight junction. Examination of the structural basis for its invasion-inhibitory action revealed 2 glycan groups on the molecule were critical for this activity.
Azithromycin 3d structure” by Giorgiogp2 – Own work. Licensed under CC BY-SA 3.0 via Commons –
Azithromycin was found to be much more efficient at inhibiting invasion than several other macrolide antibiotics and two of a panel of analogs of azithromycin exhibited improved inhibitory action. As the concentration of azithromycin required for invasion inhibition is at the upper end of what is feasible clinically, the authors suggest more efficient analogs could be developed.
The establishment of the dual action of this drug makes it a much more promising candidate for further investigation than its initially recognized, slow-acting effect, suggested. A drug that stops the parasite from entering RBCs is particularly attractive as it would both prevent the parasite from causing the symptoms of malaria and also ensure none of the stages infective to the mosquito (gametocytes) could develop and transmit the disease to new victims. It is to be hoped further development of azithromycin derivatives occurs.
Credited to Hilary Hurd
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